Journal: Biomicrofluidics

Article Title: Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow

doi: 10.1063/1.5123462

Figure Lengend Snippet: (a). Basic experimental setup showing tumor fresh media contained in syringes linked to a multiwell μ-slide III 3D chip containing either ES-2 or U87 spheroids linked through to a μ-Slide I Luer containing HUVECs and finally sample collection tubes. The experiments were carried out in a 37 °C incubator. (b). Schematic of the experimental setup. Media were flowed via either a syringe pump (constant flow) or a Kima pump (pulsatile flow) through a microfluidic chip containing tumor spheroids. MVs are released from the spheroids into the media, which is then connected to a second chip coated with HUVECs to study their interaction.

Article Snippet: Two chips were used for the HUVEC experiments, either a μ-Slide I Luer (Ibidi, Germany) or a Vena8 endothelial cell biochip (Cellix, Ireland).

Techniques:

Journal: Biomicrofluidics

Article Title: Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow

doi: 10.1063/1.5123462

Figure Lengend Snippet: TFMVs of ES-2 and U87 as a percentage of baseline values (n = 8) when perfused across a μ-Slide I Luer containing cultured HUVECs (red square) or a gelatin control chip (black circle) with no HUVECs present (n = 4) for 6 h. Error bars are SD.

Article Snippet: Two chips were used for the HUVEC experiments, either a μ-Slide I Luer (Ibidi, Germany) or a Vena8 endothelial cell biochip (Cellix, Ireland).

Techniques: Cell Culture, Control

Journal: Biomicrofluidics

Article Title: Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow

doi: 10.1063/1.5123462

Figure Lengend Snippet: Confocal microscopy of HUVECs incubated on a μ-Slide I Luer perfused with tumor media (ES-2 top panels, U87 bottom panels) with CFSE-labeled MVs. The left panels correspond to the fluorescent detection channel, middle panels are brightfield detection channel, and the right panels are the combined images.

Article Snippet: Two chips were used for the HUVEC experiments, either a μ-Slide I Luer (Ibidi, Germany) or a Vena8 endothelial cell biochip (Cellix, Ireland).

Techniques: Confocal Microscopy, Incubation, Labeling

Journal: Biomicrofluidics

Article Title: Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow

doi: 10.1063/1.5123462

Figure Lengend Snippet: (a). Basic experimental setup showing tumor fresh media contained in syringes linked to a multiwell μ-slide III 3D chip containing either ES-2 or U87 spheroids linked through to a μ-Slide I Luer containing HUVECs and finally sample collection tubes. The experiments were carried out in a 37 °C incubator. (b). Schematic of the experimental setup. Media were flowed via either a syringe pump (constant flow) or a Kima pump (pulsatile flow) through a microfluidic chip containing tumor spheroids. MVs are released from the spheroids into the media, which is then connected to a second chip coated with HUVECs to study their interaction.

Article Snippet: Microfluidic chips Two chips were used for the HUVEC experiments, either a μ-Slide I Luer (Ibidi, Germany) or a Vena8 endothelial cell biochip (Cellix, Ireland).

Techniques: